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Open Access Highly Accessed Open Badges Research article

Reverse phase chromatographic behaviour of major components in Capsicum Annuum extract

Monica Butnariu1*, Angela Caunii2 and Salomeia Putnoky3

Author Affiliations

1 Chemistry and Vegetal Biochemistry, Banat’s University of Agricultural Sciences and Veterinary Medicine from Timisoara, Calea Aradului no. 119, 300645, Timisoara, Romania

2 Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy, 2A Eftimie Murgu Square, Timisoara, 300041, Romania

3 Faculty of Medicine, Department of Hygiene, “Victor Babes” University of Medicine and Pharmacy, Bd. Victor Babes, no. 16, 300226, Timisoara, Romania

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Chemistry Central Journal 2012, 6:146  doi:10.1186/1752-153X-6-146

Published: 4 December 2012



The purpose of the study is to develop a suitable analytical method in order to establish appropriate conditions for isolation and assay of the dominant compounds in extracts of Capsicum annuum.


The studies are performed with standard substances to establish the HPLC conditions for complete separation of capsaicin and dihydrocapsaicin, the two major components of interest. Because of their prevalent apolar features, reverse phase chromatographic version was approached. Systematic studies on different eluents revealed the 65% methanol–35% water mixture as a proper mobile phase providing a complete separation of the components according to analytical claims.


The results may be of interest to develop a specific analytical procedure with advanced specificity for quantitative assay of capsaicin and dihydrocapsaicin in pharmaceutical products and in foods.

Graphical abstract