Graphite furnace atomic absorption spectrometry as a routine method for the quantification of beryllium in blood and serum

Chadi H Stephan¹ , Michel Fournier² , Pauline Brousseau² and Sébastien Sauvé¹

¹Department of Chemistry, Université de Montréal, P.O. 6128, Station Centre-Ville, Montréal, QC, Canada, H3C 3J7

²INRS-Institut Armand-Frappier, 245 Hymus, Pointe-Claire, QC, Canada H9R 3G6

author email corresponding author email

Chemistry Central Journal 2008, 2:14doi:10.1186/1752-153X-2-14


Published:	2 July 2008

Abstract

Background

A routine method for the quantification of beryllium in biological fluids is essential for the development of a chelation therapy for Chronic Beryllium Disease (CBD). We describe a procedure for the direct determination of beryllium in undigested micro quantities of human blood and serum using graphite furnace atomic absorption spectrometry. Blood and serum samples are prepared respectively by a simple 8-fold and 5-fold dilution with a Nash Reagent. Three experimental setups are compared: using no modifier, using magnesium nitrate and using palladium/citric acid as chemical modifiers.

Results

In serum, both modifiers did not improve the method sensitivity, the optimal pyrolysis and atomization temperatures are 1000°C and 2900°C, respectively. In blood, 6 μg of magnesium nitrate was found to improve the method sensitivity. The optimal pyrolysis and atomization temperatures were 800°C and 2800°C respectively.

Conclusion

In serum, the method detection limit was 2 ng l^-1, the characteristic mass was 0.22 (± 0.07) pg and the accuracy ranged from 95 to 100%. In blood, the detection limit was 7 ng l^-1, the characteristic mass was 0.20 (± 0.02) pg and the accuracy ranged from 99 to 101%.